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Multiparameter Flow Cytometry to Detect Hematogones and to Assess B... |
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by: |
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Carulli, Giovanni |
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Ottaviano, Virginia |
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Guerri, Valentina |
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Giuntini, Stefano |
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Sammuri, Paola |
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Ciancia, Eugenio M. |
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Azzara, Antonio |
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This article is from Hematology Reports[1], volume 6[2]. |
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Abstract |
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Hematogones are precursors of B-lymphocytes detected in |
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small numbers in the bone marrow. Flow cytometry is the |
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most useful tool to identify hematogones and, so far, 4- |
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color methods have been published. In addition, flow |
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cytometry is used in the diagnosis and follow-up of |
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lymphomas. We developed a flow cytometric 7-color method |
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to enumerate hematogones and to assess B-lymphocyte |
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clonality for routine purposes. We evaluated 171 cases of |
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B-cell non-Hodgkin lymphomas, either at diagnosis or in |
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the course of follow-up. By our diagnostic method, which |
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was carried out by the combination K/ |
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/CD20/CD19/CD10/CD45/CD5, we were able to detect |
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hematogones in 97.6% of samples and to distinguish normal |
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B-lymphocytes, neoplastic lymphocytes and hematogones in |
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a single step. The percentage of hematogones showed a |
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significant inverse correlation with the degree of |
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neoplastic infiltration and, when bone marrow samples not |
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involved by disease were taken into consideration, |
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resulted higher in patients during follow-up than in |
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patients evaluated at diagnosis. |
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References |
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1. //archive.org/search.php?query=journaltitle%3A%28Hematology%20Reports%29 (l… |
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2. //archive.org/search.php?query=journaltitle%3A%28Hematology%20Reports%29%20… |
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Date Published: 2014-10-17 00:33:41 |
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Identifier: pubmed-PMC4091289 |
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Item Size: 12685765 |
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Language: eng |
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Media Type: texts |
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