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Mucus protein composition of wild Channa striatus (Bloch, 1793) (Pe...
by JIPBS
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Several wild Snakehead fish or Channa striatus, with
total length and weight ranging between
24-36 cm and 130-210 g were collected from paddy field at
Kampung Selinsing, Perak. From
Bradford assay, the total protein concentration of
epidermal mucus was determined as 2.45
mg/ml. Subsequently, the samples were fractionated and
further analysed by using LC-MS/MS.
From UniProt/SwissProt and UniProt/TrEMBL databases, 192
and 157 proteins were identified.
The foremost protein detected by UniProt/SwissProt
database was keratins (21%), followed by
histones (16%), 14-3-3 proteins (13%), calmodulins (11%),
ras-related proteins (10%), creatine
kinases (8%), actins (6%) and other proteins (15%). On
the other hand, the major protein
perceived from UniProt/TrEMBL database was
uncharacterized proteins (43%), followed by
histones (21%), 60S ribosomal (8%), actins (6%), 40S
ribosomal (5%), glycolysis related
proteins (5%) and other proteins (12%). Based on the
types of protein, structural proteins (38%)
was the main protein identified by UniProt/SwissProt
database, followed by regulatory (26%),
enzymatic/catalytic (14%), calcium-binding/transport
(11%), ribosomal (6%) and contractile
proteins (5%). Whereas, protein with unknown function
(43%) was the major protein detected
by UniProt/TrEMBL database. This followed by structural
proteins (23%), ribosomals (13%),
enzymes (6%), contractiles (6%), regulatory (4%),
antioxidants (3%), and calcium-binding
protein (2%). From this study, a wide range of proteins
with various functions were detected
within the mucus of wild Channa striatus, which needs
further studies and exploration.
Date Published: 2024-05-22 09:15:22
Identifier: jipbsv-6-i-305
Item Size: 6528748
Media Type: texts
# Topics
Biological function
Channa striatus
epidermal mucus
LC-MS/MS
protein profile
Peninsular Malaysia.
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