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DTIC ADA541824: Genetic Manipulation Of Mammary Stem Cells to Recon... |
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by Defense Technical Information Center |
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This report serves as a progress report on the second |
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year of my training grant. I have completed my fifth year |
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as a graduate student in Dartmouth Medical School's |
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Department of Pharmacology and Toxicology, part of the |
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Program in Experimental Molecular Medicine (PEMM). Dr. |
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James DiRenzo serves as my mentor and chair of my thesis |
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committee. Training Tasks I completed all required |
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coursework and passed the qualifying exam, consisting of |
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a written grant proposal and its oral defense. Senior |
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members of the DiRenzo lab trained me in mouse handling, |
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histology and tumor pathology. I was also trained to |
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isolate mammary epithelial cell populations by |
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fluorescence activated cell sorting (FACS), and have |
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developed some of my own protocols for FACS-based |
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experiments. Dr. DiRenzo has also trained me in the |
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surgical technique of transplanting mammary epithelial |
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cells into cleared mammary fat pads. In the past year I |
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have also worked with our lab manager, Pratima Cherukuri, |
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M.Sc, to develop a chromatin immunoprecipitation (ChIP) |
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protocols to study the binding of transcription factors |
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and transcriptional machinery at specific loci. |
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Date Published: 2018-08-08 13:26:15 |
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Identifier: DTIC_ADA541824 |
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Item Size: 5587338 |
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Language: english |
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Media Type: texts |
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# Topics |
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DTIC Archive; ; DARTMOUTH COLL HANOVE... |
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