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DTIC ADA436963: Phosphorylation of hRad17 by atr is Required for Ce...
by Defense Technical Information Center
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Targeted protein phosphorylation is a key event that
serves to transduce DNA damage- induced cell signals from
upstream sensors to downstream effectors. ATR kinase
phosphorylates BRCA1 and Rad17 upon DNA damage. As a
first approach to evaluate the functional consequence of
IR-induced site-specifically phosphorylated residues on
BRCA1, -we have established a BRCA1-dependent
transcription-based assay which assesses the BRCA1-
dependent repression function of ZBRK1 in mammalian cell
from a reporter template bearing ZBRK1 DNA-binding sites.
I found that ZBRK1 formed a homo-tetramer on the target
gene promoter functioning as a DNA-binding dependent
repressor as well as a DNA-binding independent
corepressor. Functional dissection of ZBRK1 led to
identification and characterization of a novel BRCA1-
dependent repression domain encompassing ZBRK1 zinc
fingers 5-8 and the unique C-terminus. This C-terminal
repression domain functions in a BRCA1-dependent, histone
deactylase-dependent and promoter-specific manner.
Significantly, BRCA1 mediates ZBRK1 transcriptional
repression function by modulating- two properties of
ZBRK1 as a transcription factor: its DNA-binding and the
recruitment of other co-repressor proteins. BRCA1-
dependent ZBRK1 repression assay may now be' exploited to
evaluate the influence of IR-induced site-specific
phosphorylation of BRCA1 on its sequence-specific co-
repressor function.
Date Published: 2018-05-27 11:16:43
Identifier: DTIC_ADA436963
Item Size: 37934215
Language: english
Media Type: texts
# Topics
DTIC Archive; Tan, Wei; TEXAS UNIV HE...
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