DTIC ADA418782: Genetic Polymorphisms, Estrogens, and Breast Density | |
by Defense Technical Information Center | |
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Mammographic density appears to be a strong predictor of | |
breast cancer risk. Endogenous steroid hormones have been | |
shown to be important in the development and progression | |
of breast cancer. Because some metabolites of endogenous | |
estrogens may have more estrogenic effects than others, | |
genetically determined differences in biosynthesis and | |
metabolic pathways of estrogens may affect breast cancer | |
risk. This study will address the following specific | |
aims: 1. To examine the relation between the urinary | |
ratio of 16alpha- hydroxyestrone (16alpha-OHEl) and 2- | |
hydroxyestrone (OHEl) and polymorphisms in the genes | |
coding for some hormone metabolizing enzymes. 2.To | |
investigate differences in urinary excretion of estrone3- | |
glucuronide, a marker of endogenous estradiol, between | |
women with variant alleles for polymorphisms in genes | |
coding for hormone producing (CYP17) and metabolizing | |
enzymes (COMT, CYP1Al, CYPlA2, and CYP1B1) and women with | |
the wildtype genotype. 3. To analyze the relation of | |
mammographic density patterns with the urinary excretion | |
of estrone-3 glucuronide, the urinary ratio of 16alpha- | |
OHEl: 2%O-OHEl, and the presence of variant alleles for | |
hormone producing and metabolizing enzymes. During the | |
last year, we have completed the recruitment of 316 study | |
subjects (218 pre- and 98 postruenopausal women). The | |
subjects represent the population of Hawaii with large | |
proportions of women from different ethnic groups. The | |
majority of mammograms has undergone quantitative | |
mammographic density assessment. Blood for the DNA | |
analysis and urine samples for the premenopausal women | |
were collected on cycle day 19. Blood or mouthwash and | |
urine samples were collected for all women, coded, | |
processed, and frozen. The genotyping for 7 polymorphisms | |
using published PCR/RFLP methods has just been completed, | |
2 more are under way. The urinary analysis will be | |
finished within the next 2 months. | |
Date Published: 2018-05-14 23:47:23 | |
Identifier: DTIC_ADA418782 | |
Item Size: 20159521 | |
Language: english | |
Media Type: texts | |
# Topics | |
DTIC Archive; Maskarinec, Gertraud; H... | |
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