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DTIC ADA237035: Molecular Biology of STLV-III and HTLV-IV |
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by Defense Technical Information Center |
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We have developed a novel technique for rapidly studying |
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microheterogeneity in a population of primate |
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immunodeficiency viruses. Proviruses are rapidly cloned |
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by using an integration - competent cytoplasmic extract |
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from infected cells and integration - sensitive undersize |
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cloning vectors based on bacteriophage lambda. The |
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details of this cloning system are presented. This method |
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yields many clones of integrated proviral DNA, each of |
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which represents an independent clone of provirus |
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contained in the original infected cell population. The |
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utility of this vector to characterize in vitro |
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integration products of a primate immunodeficiency virus |
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(HIV-1) are illustrated as an example. |
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Date Published: 2018-03-02 19:47:21 |
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Identifier: DTIC_ADA237035 |
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Item Size: 4721348 |
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Language: english |
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Media Type: texts |
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# Topics |
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DTIC Archive; Sodroski, Joseph; DANA-... |
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