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DTIC ADA228229: Spatial Relationships between Drug Binding Sites on... |
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by Defense Technical Information Center |
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This report describes the preparation and/or |
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characterization of several fluorescent probes of the |
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muscle-type nicotinic acetylcholine receptor for Torpedo |
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californica. These probes include the following: (1) four |
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fluorescein isothiocyante Naja naja siamensis alpha-toxin |
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3 derivatives labeled at either lys-23, lys35, lys-49, or |
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lys-69. (2) a tetramethylrhodamine alpha- toxin |
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derivative labeled at lys-23. (3) ethidium bromide, and |
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(4) decidium. With these probes and 6-(5- |
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dimethylaminonaphthalene-1-sulfonamido)hexanoic acid |
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beta(N-trimethylammonium)ester (dansyl-C6-choline) and |
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bis(choline)-N-(4- nitrobenzo-2-oxa 1,3-diazol-7-yl)- |
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iminodiproprionate (BCNI), we estimated the distance |
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between the acetylcholine binding sites and the high- |
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affinity noncompetitive inhibitor site by using |
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fluorescence energy transfer techniques. We, also, |
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estimated the orientation of the snake venom alpha-toxin |
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bound to the acetylcholine receptor with respect to the |
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central ion channel and the plane of the membrane. (js) |
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Date Published: 2018-02-27 19:57:49 |
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Identifier: DTIC_ADA228229 |
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Item Size: 41044066 |
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Language: english |
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Media Type: texts |
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# Topics |
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DTIC Archive; Johnson, David A ; CALI... |
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