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DTIC ADA208897: The Molecular Specificity of Adsorption of Biofilm ... |
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by Defense Technical Information Center |
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Hyperimmune mouse Immunoglobulin G made against microbial |
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adhesion enhancement macromolecules (MAEM) reacts |
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specifically with plates coated with immune chicken IgG. |
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Very high titers (100,000) of hyperimmune mouse sera |
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suggest production of antibody (ies) against/MAEM |
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antigens, however, the ELISA assay could not discriminate |
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specific from apparent, nonspecific reactions. D- |
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galactose and elevated salt concentrations did not affect |
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this binding. The chicken and mouse may be making |
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antibodies to different portions of the same MAEM or to |
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different MAEM antigens. The clarify this: (1) MAEM |
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antigens from the same source are being isolated |
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independently by toad and chicken immunoaffinity columns. |
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2) Adhesion enhancement activity of the respective |
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recovered antigens is being assessed. 3) MAEM antigens |
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recovered from the toad immunoaffinity column are being |
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examined for their quantitative recovery and adhesion |
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activity when they are reacted with the immunoaffinity |
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column made with chicken antibodies. 4) Antigens |
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recovered by the two types of columns will be used to |
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produce hyperimmune mouse sera for reexamination of the |
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ELISA specificity. Inhibitory effects of these sera on |
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the adhesion enhancement activity of MAEM antigens will |
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be used to assess similarities among adsorbed MAEM |
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antigens. |
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Date Published: 2018-02-22 20:18:36 |
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Identifier: DTIC_ADA208897 |
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Item Size: 4598349 |
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Language: english |
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Media Type: texts |
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