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DTIC ADA204368: Immune Response of Humans to the Circumsporozoite P... |
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by Defense Technical Information Center |
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Humans naturally exposed to malaria make antibodies to |
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the repeat region of the circumsporozoite (CS) protein. A |
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recombinant DNA and a synthetic peptide subunit vaccine |
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derived from the CS protein of Plasmodium falciparum have |
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shown promise in the protection against experimental, |
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sporozoite induced malaria in humans. Human antibody |
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response to immunization with these vaccines has been |
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inconsistent. A possible explanation for the inconsistent |
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response of humans immunized is that there is some |
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genetic restriction of the human T cell response to |
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(NANP)n. To determine if a T cell epitope on the repeat |
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region stimulated T cell help for this antibody, we used |
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R32tet32, a recombinant construct derived from the repeat |
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region of the circumsporozoite protein of P. falciparum, |
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to stimulate in vitro mononuclear cells from residents of |
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an area hyperendemic for malaria. Three groups differing |
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in the length of time they had resided in a malarious |
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area were studied. The percentage of individuals in each |
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group who had positive antibody responses to R32tet32 |
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increased with increased exposure to malaria. Antibody |
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positivity was not correlated with in vitro lymphocyte |
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proliferation responses to the antigen. Lymphocytes from |
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79% of the individuals showing serum antibodies to |
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R32tet32 failed to respond in a lymphocyte transformation |
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assay, suggesting that T cell helper activity in these |
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individuals was based upon the recognition of a T cell |
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epitope not located within this peptide. Reprints. |
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Date Published: 2018-02-21 23:31:17 |
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Identifier: DTIC_ADA204368 |
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Item Size: 5046933 |
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Language: english |
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Media Type: texts |
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# Topics |
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DTIC Archive; Campbell, James R ; NAV... |
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