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DTIC ADA203018: Cloning and Expression of Genes for Dengue Virus Ty... |
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by Defense Technical Information Center |
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Using oligodeoxynucleotide primers, we cloned the region |
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of DEN-2 RNA encoding the structural proteins C, prM (M), |
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and E glycoprotein. The cDNA clones were completely |
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sequenced. Using a synthetic primer complementary to the |
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3'-end of DEN-2 RNA, additional cDNA clones, mapping 3' |
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to the previously characterized clones, A4 and B2 were |
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isolated. Genome 'walking' using these cDNA clones as |
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probes, pPM-F12, pPM-A10, pPM53 were isolated, all |
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mapping toward the 3'-end of the genome. These cDNA |
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clones were sequenced. To complete the cDNA cloning of |
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DEN-2 genome, a novel approach was used which involved |
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the polymerase chain reaction. Using this approach, the |
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sequences between nucleotide 9950 and the 3'- terminus |
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were amplified in a Taq polymerase-catalyzed chain |
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reaction. The amplified DNA was cloned and sequenced. |
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Keywords: Dengue, Vaccines, Diagnosis medicine. |
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Date Published: 2018-02-21 19:55:51 |
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Identifier: DTIC_ADA203018 |
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Item Size: 23879832 |
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Language: english |
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Media Type: texts |
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# Topics |
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DTIC Archive; Padmanabhan, Radha K ; ... |
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