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15Jan92 About Gopher Materials & Methods
3
15Jan92 GENERAL INFORMATION
e
15Jan92 EXO III SEQUENCING DELETIONS
1
15Jan92 SEQUENCING DOUBLE-STRANDED DNA
f
15Jan92 Colony lifts
9
15Jan92 CsCl/Et Br plasmid isolation procedure
3
15Jan92 Isolation of total RNA using CsCl/EtBr gradients
3
15Jan92 DMS-treating DNA
7
15Jan92 DNase I footprinting
3
15Jan92 dscDNA synthesis
d
15Jan92 End-labeling DNA fragments
3
15Jan92 End-labeling RNA
5
15Jan92 Ethidium bromide spot test for DNA or RNA concentration…
c
15Jan92 Eubacterial RNA isolation
f
15Jan92 EXONUCLEASE III FOOTPRINTING
8
15Jan92 THE FIGURE-EIGHT TEST
a
15Jan92 Giant plasmid mini-prep
8
15Jan92 Genomic DNA isolation
f
15Jan92 GLYOXAL GELS
d
15Jan92 Hybridization procedure #2
3
15Jan92 Preparing 5' end-labeled alkaline hydrolyzed RNA
b
15Jan92 5' End-labeling Alkaline Hydrolyzed rRNA
e
15Jan92 rRNA sequencing using reverse transcriptase
7
15Jan92 ISOLATING M13 CLONES AND PREPARING INFECTED CULTURES
6
15Jan92 Molecular Cloning Manual (Maniatis)
9
15Jan92 Culturing Methanogens
b
15Jan92 FERMENTING METHANOGENS
8
15Jan92 Micro-protein assay
a
15Jan92 Precipitation of high MW RNAs by NaCl
5
15Jan92 NICK TRANSLATION
2
15Jan92 Northern gels
8
15Jan92 NITROCELLULOSE FILTER HYBRIDIZATION
7
15Jan92 OLIGO(dT)-CELLULOSE CHROMATOGRAPHY
0
15Jan92 32P-pCp 3' End-labeling RNA
3
15Jan92 POLY(A) ASSAY USING 3H-POLY(U)
5
15Jan92 POLY(U)-SEPHAROSE CHROMATOGRAPHY
7
15Jan92 P-RNA purification
4
15Jan92 SEQUENCING GELS
b
15Jan92 SINGLE STEP PROKARYOTIC RNA ISOLATION
e
15Jan92 SOUTHERN BLOT PROTOCOL #2
9
15Jan92 PREPARING SPOT BLOTS
e
15Jan92 SEPHADEX G-50 SPIN COLUMN
f
15Jan92 ssDNA from pTZ/pBLUESCRIPT
6
15Jan92 Standard ligation
d
15Jan92 T7 run-off transcription
b
15Jan92 TAB restriction buffer
4
15Jan92 TRANSFORMATION OF CaCl2 TREATED E. COLI CELLS WITH PLA…
1
15Jan92 Fixing DNA or RNA to membrane filter with UV
1
15Jan92 Saccharromyes cerevisiae TOTAL RNA ISOLATION
1
15Jan92 MINI CLEARED LYSATE PROTOCOL
d
15Jan92 OLIGO(dT) CELLULOSE BATCH CHROMATOGRAPHY
7
15Jan92 32P-cDNA SYNTHESIS
