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March 2015 – ISS On-Orbit Status Report [1]

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Date: 2022-09

Human Research Program (HRP) Collections: Kelly performed a suite of blood and saliva collections for the Microbiome, Salivary Markers, and Twins Study experiments. He collected saliva and placed the collections into Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI) for a week in support of Microbiome and Salivary markers. Today’s blood collection satisfies both the Flight Day 7 (FD7) and FD10 requirements for both experiments.

Salivary Markers experiment aims to identify if there are any risks of an adverse health event in crewmembers due to an impaired immune system. Maintaining a robust immune system during prolonged spaceflight missions is important to ensure mission success. If any impairment in immunity is found due to spaceflight, this will allow the development of appropriate countermeasures to help mitigate the risks.

The Microbiome experiment investigates the impact of space travel on both the human immune system and an individual’s microbiome (the collection of microbes that live in and on the human body at any given time).

Gene, Immune and Cellular Responses to Single and Combined Space Flight Conditions-B (Triplelux-B): Following the successful completion of the Triplelux-B experiment, Cristoforetti removed the Biolab video tape, uninstalled the Automatic Ambient Stowage (AAS), and the Experiment Containers (ECs) from the Biolab facility. The video tape will be returned for ground analysis. Biolab will be configured for Triplelux-A which flies on SpaceX-6 launching next week. The objective of Triplelux-A is to investigate and compare the ability of hemocytes (from the blue mussel) to kill pathogens under normal gravity and microgravity condition. The goal is to quantify the anti-microbial action of the hemocytes, using the chemical reaction that results in emission of light. This light will be detected with photomultipliers. The experiment might lead to a better understanding of immune system depression in spaceflight.

Image Processing Unit (IPU) Hard Disk Drive (HDD) Remove and Replace (R&R): Cristoforetti R&Rd the IPU in the Ryutai Rack. On GMT 84 (March 25, 2015) Japan Aerospace Exploration Agency (JAXA) ground controllers encountered a problem downloading Dynamic Surf-2 images. Initial troubleshooting steps included rebooting the hardware and rack. Ground teams are evaluating the status of the R&R and will have more data by GMT 92 (April 2).

Aniso Tubule: Cristoforetti retrieved the Aniso Tubule from the +4°C MELFI drawer and initiated a light treatment, exposing the sample to the cabin’s ambient light environment. She then inserted the Aniso sample into the Cell Biology Experiment Facility (CBEF). The Aniso Tubule investigation seeks to understand the mechanisms that plants use in growing stems of the right thickness to support them against gravity. Microtubules, part of a plant cell’s molecular skeleton, align themselves with the local gravity field and thus produce short, thick bodies in hypergravity. This research will germinate Arabidopsis hypocotyls in space and then use a fluorescence microscope in the Kibo module to analyze the directions taken by tubules in the stem just below the leaves of new seedlings.

Radiation Dosimetry Inside ISS-Neutrons 2 (RADI-N2): Cristoforetti collected the eight neutron bubble detectors and returned them to Shkaplerov. This investigation is to better characterize the ISS neutron environment and define the risk posed to the crewmembers’ health and provide the data necessary to develop advanced protective measures for future space flight. For each session 8 detectors are initialized and deployed in predetermined locations for measurements: a set of 6 spectrometric detectors and two control ones are placed on a wall in the ISS Segment. After 7 days the detectors are collected and read using the BUBBLE reader.

Node 3 (N3) Carbon Dioxide Removal Assembly (CDRA) Status: Yesterday N3 CDRA shut down when Air Selector Valve (ASV)104 failed to transition from position A to B. Attempts to manually command the valve were unsuccessful. The Ground ran an Active Cool Down procedure for 30 minutes. ASV104 is now responding to commands and nominal N3 CDRA operations have been resumed.

On-Board Training (OBT): Virts and the newly arrived 42S crew reviewed portable emergency equipment locations for gas masks and fire extinguishers. When finished, they demonstrated/evaluated the ability to communicate with Mission Control Centers (MCC) while wearing emergency masks.

SpaceX-6 (SpX-6) Preparation: In preparation for SpX-6 launch scheduled for April 13, Robotics Ground Controllers are currently performing a Mobile Transporter (MT) translate from Work Site (WS) 2 to WS4.

Today’s Planned Activities

All activities were completed unless otherwise noted.



TWIN – Sample Collection and Prep for Stowage

HRF – Sample MELFI Insertion

HRF – Preliminary steps before blood sample collection

HRF – Blood collection assistance

Verification of anti-virus scan results on Auxiliary Computer System (ВКС) laptops / r/g 8247

BIOSIGNAL. Temperature check in Cryogem-03 / r/g 8342

HRF Refrigerated Centrifuge Configuration

Regeneration of БМП Ф2 Micropurification Cartridge (start)

Periodic fitness evaluation – subject

Private Exercise Conference with exercise specialist

CASKAD. Manual Mixing in Bioreactor / r/g 8358

TPLXB. Unstow Hardware

HRF – Blood Sample Collection Closeout Ops

Locating unit 800А No.2395121201

Deactivation of ТК 716 Gas Analyzer

CARDIOVECTOR. Experiment Ops. / r/g 8374

HRF – Urine Sample MELFI Insertion

HRF Blood Collection Hardware Stowage

ТК 716 Transfers and IMS Ops / r/g 8356

CIR Alignment Guide Installation

CARDIOVECTOR. Photography of the Experiment Ops / r/g 8373

Image Processing Unit (IPU) System Hard Disk Drive R&R

Installation of Easily Detachable Cargo r/g 8371

Deactivation of ТК 715 Gas Analyzer

ANISO – Retrieval of Ziploc bags with samples from MELFI

Medical Ops OBT

MATRYOSHKA-R. r/g 8372

SEISMOPROGNOZ r/g 8267

ANISO – Attaching Ziploc bags with samples for exposure

VHF1 Comm check from RS with MCC (Houston, Huntsville, Munich, Tsukuba, Moscow) via US Ground Sites)

TV Greetings r/g 8376

OBT Emergency Hardware Familiarization

IDENTIFIKATSIYA r/g 8239

TPLXB. Video Tape Removal

TPLXB. Insert Removal and Reconfiguration

TPLXB. Container Reconfiguration

CUCU – Activation

Emergency Mask Drill

NANO – Routing Ethernet Cable

WRS – Recycle Tank Fill [Aborted]

CUCU Check

MATRYOSHKA-R. Installation r/g 8370

Crew time for ISS adaptation and orientation

MATRYOSHKA-R. BUBBLE-dosimeter collection and measurements. / r/g 8369

TPLXB. Hardware Stowage for Final Experiment

Stem Cell Differentiation (SCD) – Hardware Temperature Check

RADIN – Removal of Bubble-Detectors

IMS Delta File Prep

MATRYOSHKA-R. Handover of BUBBLE-dosimeter detectors from USOS. / r/g 8369

RADIN – Handover of Bubble-Detectors

MATRYOSHKA-R. BUBBLE-dosimeter collection and measurements. / r/g 8369

CUCU – Temporary Stowage

WRS Sample Collection

Photo of a scuff mark left by the Active Docking Mechanism probe on the MRM2 r/g 2664

Docking Cone Internal Surface Photos Downlink

WRS – Recycle Tank Fill [Aborted]

Crew time for ISS adaptation and orientation

Dragon Pre-pack for disposal

Preventive maintenance of MRM2

TOCA Potable Water Dispenser (PWD) Sample Analysis

ISS Crew Adaptation

Crew time for ISS adaptation and orientation

Hematocrit Hardware Setup

HMS Defibrillator Inspection

Water processing using MCD

SEISMOPROGNOZ. Downlink data from Control and Data Acquisition Module r/g 8267

ANISO – Sample Insertion into bags

CASKAD. Manual Mixing in Bioreactor / r/g 8358

TOCA Data Recording

Regenegation of Micropurification unit

Completed Task List Items

CHeCS hardware relocate

Ground Activities

All activities were completed unless otherwise noted.



CUCU checkout

MSS powerup

MBS camera checkout

MT pre-translation checkout

MT pre-translation survey

MT translation from WS2 to WS4 [In Work]

MSS powerdown [Planned later this evening]

Three-Day Look Ahead:

Wednesday, 04/01: Ocular Health, water quality monitoring, T2 quarterly inspection, SSRMS walkoff to N2

Thursday, 04/02: Ocular Health, CIR imaging reconfig, WHC manual fill

Friday, 04/03: Ocular Health, ER7 cable connection, Rodent Research s/w load, Recycle tank QD reconfig

QUICK ISS Status – Environmental Control Group:

[END]
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[1] Url: https://blogs.nasa.gov/stationreport/2015/03/

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