mRNA Vaccines Alter Human Liver DNA In Vitro

Pfizer’s BioNTech vaccine causes intracellular reverse transcription
of BNT162b2 mRNA into human DNA in vitro, renewing concerns
that vaccines may introduce spike protein into the nuclei of cells
(https://bit.ly/3qASVsP).
The findings emerged Friday in a peer-reviewed article entitled
“Intracellular Reverse Transcription of Pfizer BioNTech COVID-19
mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line” in
the Current Issues in Molecular Biology Journal, an imprint of MDPI,
the largest open-access publisher in the world and the fifth-largest
publisher overall in terms of journal paper output.
Researchers Warn: Pfizer Vaccine May Affect Integrity of Genomic
DNA “Our study shows that BNT162b2 can be reverse transcribed to
DNA in liver cell line Huh7, and this may give rise to the concern
if BNT162b2-derived DNA may be integrated into the host genome
and affect the integrity of genomic DNA, which may potentially
mediate genotoxic side effects,” the authors warn.
The study, authored by a team of Swedish researchers at Lund
University, concluded that Pfizer’s COVID-19 mRNA vaccine entered
the human liver cell line Huh7 in vitro and BNT162b2 mRNA was
subsequently transcribed intracellularly into DNA within six hours
of exposure.
An “Immortal” Human Cell Line
The Huh7 cell line is a permanent line of liver cells derived from
male hepatoma tissue that was surgically removed from a 57-year-old
Japanese man in 1982. For the next 40 years, Huh7 and its derivatives
were used in thousands of laboratories across the planet as a
convenient experimental substitute for primary hepatocytes.
Hepatocytes, the major parenchymal cells in the liver, play pivotal
roles in metabolism, detoxification, and protein synthesis.
Hepatocytes also activate innate immunity against invading
microorganisms by secreting innate immunity proteins.
In Vitro Vs. In Vivo Caveats
Researchers who conduct in vitro studies commonly remind that
results that emerge from laboratories and test tubes often differ
from results which are derived in living, fully intact organisms.
And the Huh7, itself, has limitations that could introduce errors
or anomalies into laboratory results.
Still, the study conducted by researchers at one of Europe’s oldest
and most prestigious research institutions raises serious questions
about Pfizer’s mRNA vaccines’ impact on human DNA, which
have yet to be subjected to the typical years-long (or decades-long)
battery of long-term safety monitoring protocols.
Changes in Gene Expression of LINE-1
The study authors exposed Huh7 cells to Pfizer’s BNT162b2 mRNA
and then performed a quantitative polymerase chain reaction on RNA
extracted from the cells. The research team discovered high levels
of BNT162b2 in Huh7 cells and changes in gene expression of long
interspersed nuclear element-1, or LINE-1, which is an endogenous
reverse transcriptase.
BNT162b2 mRNA Reverse Transcribed Into DNA Within Six Hours
Of Exposure
The authors conclude that BNT162b2 transfects into human liver cell
line huh7 in vitro, altering LINE-1 expression and distribution. The
authors also find that “BNT162b2 mRNA is reverse transcribed
intracellularly into DNA in as fast as six hours upon BNT162b2
exposure.”
BNT162b2 is a lipid nanoparticle (LNP)–encapsulated, nucleoside
modified RNA vaccine (modRNA) which resembles gene therapy
platforms. Pfizer’s mRNA vaccine encodes the full-length of
SARS-CoV-2 spike protein. That spike protein is modified by two
proline mutations to ensure antigenically optimal pre-fusion
conformation, mimicking the intact virus to elicit virus-neutralizing
antibodies.
A recent study showed that SARS-CoV-2 RNAs can be reverse
transcribed and integrated into the genome of human cells, which
the authors said led them to investigate whether spike transfected
by mRNA vaccines might have comparable effects.
CDC Says mRNA Vaccines Does Not Enter Nuclei or Interact With DNA
mRNA vaccines were not designed to invade human cells’ nuclei.
In fact, the United States Centers for Disease Control claimed in
December that “COVID-19 vaccines do not change or interact with
your DNA in any way.”
“The genetic material delivered by mRNA vaccines never enters the
nucleus of your cells, which is where your DNA is kept,”
the government agency website said. “Viral vector COVID-19
vaccines deliver genetic material to the cell nucleus to allow our
cells to build protection against COVID-19. However, the vector
virus does not have the machinery needed to integrate its genetic
material into our DNA, so it cannot alter our DNA.”
The Nucleus is the “Brain” of Human Cells, Site of DNA Replication
Some biologists refer to the nucleus, metaphorically, as the “brain”
of the cell. The nucleus is the most prominent of cells’ organelles and
contain genetic information in the form of deoxyribonucleic acid
(DNA) and is the site of DNA replication. The nucleus is also the
site for the synthesis of ribonucleic acid (RNA) which is the
template for synthesis of other cell proteins and for protein factories
of the cell called ribosomes.
Transfection of spike protein into the nucleus opens the possibility
of DNA modification, the authors wrote.
mRNA Vaccines Effect on “Genomic Integrity” Should Be Studied
“At this stage, we do not know if DNA reverse transcribed from
BNT162b2 is integrated into the cell genome,” the authors wrote.
“Further studies are needed to demonstrate the effect of BNT162b2
on genomic integrity, including whole genome sequencing of cells
exposed to BNT162b2, as well as tissues from human subjects who
received BNT162b2 vaccination.”

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