Introduction
Introduction Statistics Contact Development Disclaimer Help
Return Create A Forum - Home
---------------------------------------------------------
Iranian Tissue Engineering
https://ite.createaforum.com
---------------------------------------------------------
*****************************************************
Return to: Cardiovascular Tissue Engineering
*****************************************************
#Post#: 113--------------------------------------------------
Chemically defined generation of human cardiomyocytes
By: SSamani Date: March 10, 2015, 1:10 am
---------------------------------------------------------
[font=verdana]
[center]Chemically defined generation of human
cardiomyocytes[b][/center]
[center]Nature Methods Vol 11, Issue 8, 2014
(DOI:10.1038/nmeth.2999)[/center]
Existing methods for human induced pluripotent stem cell (hiPSC)
cardiac differentiation are efficient but require complex,
undefined medium constituents that hinder further elucidation of
the molecular mechanisms of cardiomyogenesis. Using hiPSCs
derived under chemically defined conditions on synthetic
matrices, we systematically developed an optimized cardiac
differentiation strategy, using a chemically defined medium
consisting of just three components: the basal medium RPMI 1640,
L-ascorbic acid 2-phosphate and rice-derived recombinant human
albumin. Along with small molecule�based induction of
differentiation, this protocol produced contractile sheets of up
to 95% TNNT2+ cardiomyocytes at a yield of up to 100
cardiomyocytes for every input pluripotent cell and was
effective in 11 hiPSC lines tested. This chemically defined
platform for cardiac specification of hiPSCs will allow the
elucidation of cardiomyocyte macromolecular and metabolic
requirements and will provide a minimal system for the study of
maturation and subtype specification.
[center][b][size=12pt]Download link
http://www.uplooder.net/cgi-bin/dl.cgi?key=e426918ec25b53da4b5478d17f7b1e8f[/ce…
*****************************************************
You are viewing proxied material from gopher.createaforum.com. The copyright of proxied material belongs to its original authors. Any comments or complaints in relation to proxied material should be directed to the original authors of the content concerned. Please see the disclaimer for more details.