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Return to: Ocular Tissue Engineering |
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#Post#: 87-------------------------------------------------- |
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Corneal repair by iPSCs and amphiphatic chitosan hydrogel |
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By: SSamani Date: February 21, 2015, 2:16 pm |
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[font=verdana][center]Corneal repair by human corneal |
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keratocyte-reprogrammed iPSCs and amphiphatic |
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carboxymethyl-hexanoyl chitosan hydrogel |
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Biomaterials 33 (2012) 8003-8016[/center] |
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Abstract |
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Induced pluripotent stem cells (iPSCs) have promising potential |
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in regenerative medicine, but whether iPSCs can promote corneal |
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reconstruction remains undetermined. In this study, we |
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successfully reprogrammed human corneal keratocytes into iPSCs. |
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To prevent feeder cell contamination, these iPSCs were cultured |
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onto a serum- and feeder-free system in which they remained |
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stable through 30 passages and showed ESC-like pluripotent |
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property. To investigate the availability of iPSCs as |
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bioengineered substitutes in corneal repair, we developed a |
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thermo-gelling injectable amphiphatic carboxymethyl-hexanoyl |
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chitosan (CHC) nanoscale hydrogel and found that such gel |
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increased the viability and CD44+proportion of iPSCs, and |
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maintained their stem-cell like gene expression, in the presence |
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of culture media. Combined treatment of iPSC with CHC hydrogel |
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(iPSC/CHC hydrogel) facilitated wound healing in surgical |
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abrasion-injured corneas. In severe corneal damage induced by |
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alkaline, iPSC/CHC hydrogel enhanced corneal reconstruction by |
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downregulating oxidative stress and recruiting endogenous |
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epithelial cells to restore corneal epithelial thickness. |
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Therefore, we demonstrated that these human |
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keratocyte-reprogrammed iPSCs, when combined with CHC hydrogel, |
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can be used as a rapid delivery system to efficiently enhance |
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corneal wound healing. In addition, iPSCs reprogrammed from |
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corneal surgical residues may serve as an alternative cell |
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source for personalized therapies for human corneal damage. |
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[center][size=12pt]Download link |
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http://www.uplooder.net/cgi-bin/dl.cgi?key=9bb289049982f5c50303fbc43602921e[/ce… |
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