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Iranian Tissue Engineering |
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Return to: Scientific resources and Lab. Skils |
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#Post#: 70-------------------------------------------------- |
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A Method for Rapid Demineralization of Teeth and Bones |
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By: nourani Date: January 29, 2015, 7:12 pm |
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[center]A Method for Rapid Demineralization of Teeth and |
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Bones[/center] |
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Andrew Cho, Shigeki Suzuki, [...], and Ashok B Kulkarni |
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In summary, we have optimized the conditions for rapid |
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demineralization of mouse skulls, which significantly shortens |
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the time required for complete demineralization but does not |
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affect the enzyme activity or nucleic acids in the tooth |
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sections. We recommend 0.1M EDTA as a safe decalcifier, to be |
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used at 42�C for successful demineralization of mouse skulls. |
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These conditions could be widely applied for the enzymatic and |
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immunohistochemical analysis of other proteins in hard tissues. |
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Moreover, rapid demineralization using this method will promote |
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quicker analysis of genes and proteins implicated in tooth and |
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bone diseases. |
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#Post#: 81-------------------------------------------------- |
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Re: A Method for Rapid Demineralization of Teeth and Bones |
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By: SSamani Date: February 13, 2015, 8:07 am |
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Full article. |
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[center]Download link |
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http://www.uplooder.net/cgi-bin/dl.cgi?key=2d696d6c9d78d6819bbe99d0428cb134[/ce… |
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