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Return to: Bioactive Glass in Tissue Engineering |
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#Post#: 28-------------------------------------------------- |
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Bioglass application in Peripheral Nerve Regeneration |
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By: nourani Date: December 17, 2014, 7:47 pm |
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Development of a Nerve Growth Conduit for Peripheral Nerve |
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Regeneration |
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Mohammad Reza Nourani1* |
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1Tissue Engineering Research Center, Applied Biotechnology |
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Research Center, Baqiyatallah University of Medical Sciences, |
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Tehran, Iran |
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*Corresponding author: Mohammad Reza Nourani, PhD, Tissue |
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Engineering Research Center, Applied Biotechnology Research |
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Center, Baqiyatallah Hospital, Baqiyatallah University of |
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Medical Sciences, Mollasadra Ave, Tehran, Iran. Tel: +98 21 8821 |
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1523; Fax: +98 21 8821 1524; E-mail: [email protected] |
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In peripheral nerve injuries, the gap between the proximal and |
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distal ends of the injured nerve should be bridged by a |
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tube-shape implant in order to guide the sprouts sent out by the |
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proximal end to the distal end. Application of both autografts |
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and allografts are limited; the former by availability of donor |
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tissue, donor site morbidity and formation of neuroma; and the |
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latter by its low potential in promotion of nerve regeneration |
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and the risk of transmission of pathogens. Therefore, conduits |
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made of different biomaterials have been developed to regenerate |
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a nerve gap wider than 10 mm. In this study, different |
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biomaterial solutions were prepared. Then, a mandrel was soaked |
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in the solutions and freeze-dried. The conduits underwent SEM |
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for assessment of pores size and surface morphology and FTIR for |
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evaluation of chemical structure. Also, MTT assay was performed |
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using rat mesenchymal stem cell lines to test for cytotoxicity. |
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A final histopathological study was performed too. The results |
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showed that the conduits had a highly porous fine structure with |
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interconnected and partly equal-sized pores. FTIR spectra showed |
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presence of ordinary functional groups. In vitro studies |
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confirmed the ability of the conduits to support growth of |
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fibroblast cells and histopathological study revealed the |
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growing processes of nerve fibers from the proximal end of the |
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cut nerve to its distal end. It could be concluded that the |
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nerve growth conduit is suitable for guiding axonal growth and |
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supporting peripheral nerve regeneration. In this presentation, |
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different types of nerve conduits are explained. |
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http://onlinelibrary.wiley.com/doi/10.1111/aor.12136/full |
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http://onlinelibrary.wiley.com/doi/10.1111/aor.12136/full |
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